000 05901cam a2200637Ia 4500
001 ocn881571700
003 OCoLC
005 20190328114807.0
006 m o d
007 cr cnu---unuuu
008 140623s2014 onc o 000 0 eng d
040 _aEBLCP
_beng
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019 _a961202138
_a1076563831
020 _a9780124095366
_q(electronic bk.)
020 _a0124095364
_q(electronic bk.)
020 _a9780124104907
_q(electronic bk.)
020 _a0124104908
_q(electronic bk.)
020 _z9780124095366
020 _z0124095364
035 _a(OCoLC)881571700
_z(OCoLC)961202138
_z(OCoLC)1076563831
050 4 _aQH442.6
060 4 _aQY 25
072 7 _aSCI
_x013060
_2bisacsh
072 7 _aTEC
_x009010
_2bisacsh
082 0 4 _a660.65
_223
245 0 0 _aTransgenic animal technology : a laboratory handbook /
_h[electronic resource]
_cedited by Carl A. Pinkert.
250 _a3rd ed.
260 _aBurlington :
_bElsevier Science,
_c2014.
300 _a1 online resource (715 pages).
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
490 1 _aElsevier insights
588 0 _aPrint version record.
520 _aTransgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer - from molecular methods to whole animal considerations - for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engine.
505 0 _aFront Cover; Transgenic Animal Technology; Copyright Page; Contents; List of Contributors; Preface; One: Overview; 1 Introduction to Transgenic Animal Technology; I. Introduction; II. Historical Background; III. Applications and Overview of Text; References; Two: Transgenic Animal Production Focusing on the Mouse Model; 2 DNA Microinjection, Embryo Handling, and Germplasm Preservation; I. Introduction; II. General Methods; A. DNA Preparation and Purification; 1. DNA Construct/Fragment Structure; 2. Factors that Influence Transgenic Mouse Production; 3. Preparation of DNA for Microinjection.
505 8 _aA. Construction and Isolation of DNAb. DNA Purification; c. Purification and Quantification of DNA; B. Superovulation, Egg Culture, and Harvest; 1. Mouse Strains for Microinjection and Transgenic Models; a. Strains; b. Genetic Variability and Transgene Expression; 2. Superovulation of Mice; a. Pregnant Mare Serum Gonadotropin and Human Chorionic Gonadotropin; b. Follicle Stimulating Hormone and Luteinizing Hormone Delivered with Osmotic Pumps; 3. Production of Eggs from Superovulated Females; a. Colony; b. Influence of Litter Number; 4. In Vitro Culture of Superovulated Eggs; a. Equipment.
505 8 _aB. Atmosphere for Culturing Eggsc. Glass Pipettes for Manipulating Eggs; d. Media; e. Water Sources; f. Culture Dishes; 5. Harvesting Superovulated Eggs; a. Mating; b. Oviduct Dissection; c. Release of Eggs from the Oviduct; d. Egg Development and Microinjection Timing; e. Efficiency of Egg Culture; C. Microinjection Needles and Slides; 1. Types of Glass; 2. Preparation of Glass; 3. Pipette Pullers; 4. Injection Pipette Shapes; 5. Beveling Hollow Capillary Microinjection Needles; 6. Microforge Shaping of Pipettes; a. Manufacturers; b. Heating Filament; c. Holding Pipette; d. Injection Pipette.
505 8 _a7. Microinjection Slide for Eggsa. Slide Designs; b. Microinjection Media; c. Arrangement of DNA and Media Drops; D. Microinjection Equipment; 1. Microscopes; 2. Micromanipulators; a. Micropipette Holders; b. Micromanipulator Adjustments; c. Antivibration Tables; 3. Microinjection Systems; a. General; b. Oil-Driven Injection Assembly; 4. Oil-Driven Holding Pipettes; 5. Electronic Delivery Systems; E. Microinjection Procedure; 1. Preparation of Injection and Holding Pipettes (Micrometer Syringe System); a. Holding Pipette; b. Injection Pipette; c. Aligning the Injection Needle.
505 8 _aD. Loading DNA into the Injection Needle2. Preparing Eggs for Microinjection; 3. Steps for Efficient Manipulation of Eggs During Microinjection; a. General; b. Micromanipulators; c. Microinjection Needle; d. Controls and Egg Manipulation; 4. Visualizing and Injecting the Egg Pronuclei; a. Orientation; b. Egg Membrane Penetration with the Injection Needle; c. Filling the Pronuclei with DNA Solution; 5. Criteria for Microinjection to Yield Transgenic Mice; a. Treatment After Microinjection; b. Egg Lysis; c. Record Keeping; d. In Vitro Culture of Microinjected Eggs; F. Egg Transfer.
650 0 _aAnimal genetic engineering.
650 0 _aTransgenic animals.
650 0 _aAnimal biotechnology.
650 7 _aSCIENCE
_xChemistry
_xIndustrial & Technical.
_2bisacsh
650 7 _aTECHNOLOGY & ENGINEERING
_xChemical & Biochemical.
_2bisacsh
650 7 _aAnimal biotechnology.
_2fast
_0(OCoLC)fst00809103
650 7 _aAnimal genetic engineering.
_2fast
_0(OCoLC)fst00809192
650 7 _aTransgenic animals.
_2fast
_0(OCoLC)fst01154666
650 2 _aAnimals, Genetically Modified.
655 4 _aElectronic books.
655 2 _aLaboratory Manuals.
700 1 _aPinkert, Carl A.,
_eeditor.
776 0 8 _iErscheint auch als:
_nDruck-Ausgabe
_aPinkert, Carl A. Transgenic Animal Technology .
_tA Laboratory Handbook
830 0 _aElsevier insights.
856 4 0 _3ScienceDirect
_uhttp://www.sciencedirect.com/science/book/9780124104907
999 _c246920
_d246920