04741cam a2200529Ii 4500 ocn945376540 OCoLC 20190328114814.0 m o d cr bn|---a|cuu 160322s2016 enka ob 000 0 eng d OPELS eng rda pn OPELS UIU YDX U3W STF D6H AU@ OCLCQ OCLCO 957680214 9780128005101 0128005106 9780128002766 012800276X (OCoLC)945376540 (OCoLC)957680214 RB155.8 QZ 50 615.8/95 23 Adenoviral vectors for gene therapy / [electronic resource] edited by David T. Curiel. 2nd ed. Londonm UK : Academic Press is an imprint of Elsevier, 2016. 1 online resource : illustrations text txt rdacontent computer c rdamedia online resource cr rdacarrier Includes bibliographical references. Online resource; title from PDF title page (ScienceDirect, viewed March 22, 2016). Front Cover; Adenoviral Vectors for Gene Therapy; Adenoviral Vectors for Gene Therapy; Copyright; Contents; List of Contributors; 1 -- Adenovirus Structure; 1. Historical Perspective on Adenovirus Structure; 2. Hexon Structure and Capsid Packing; 3. Penton Base Structure and Integrin-Binding RGD Loop; 4. Fiber Structure and Receptor Interactions; 5. Atomic Resolution Cryo-Electron Microscopy and X-ray Crystallographic Adenovirus Structures; 6. Hexons in the Atomic Resolution Adenovirus Structures; 7. Conformational Differences of the Penton Base in the Atomic Resolution Adenovirus Structures. 8. Alternate Assignments for the Four-Helix Coiled Coil9. Protein IIIa Structure; 10. Protein IX Structure; 11. Core Protein V Structure; 12. Protein VI Structure; 13. Protein VIII Structure; 14. Adenovirus Protease; 15. Concluding Remarks; References; 2 -- Biology of Adenovirus Cell Entry: Receptors, Pathways, Mechanisms; 1. Introduction; 2. Entry Pathways: Impact of Capsid Proteins; 3. Attachment Factors and Signaling Receptors; 3.1 Accessing the CAR; 3.2 Using CD46 in High- and Low-Affinity Modes; 3.3 Desmoglein-2; 3.4 Sialic Acid; 3.5 Heparan Sulfate. 3.6 Scavenger Receptors and Other Attachment Factors3.7 Internalization Co-receptors: Integrins; 3.8 Extracellular Factors Influencing Viral Tropism; 3.8.1 Coagulation Factors; 3.8.2 Immunoglobulins and Complement; 3.8.3 Lactoferrin; 3.8.4 Dipalmitoylphosphatidylcholine; 4. Endocytosis; 4.1 Clathrin-Mediated Endocytosis; 4.2 Macropinocytosis; 4.3 Phagocytosis; 4.4 Caveolar Endocytosis; 5. Endosomal Escape; 5.1 Protein VI for Membrane Lysis; 5.2 A New Concept: Hijacking Membrane Repair for Endosome Lysis; 6. Targeting the Nucleus; 6.1 Transport through the Cytoplasm. 6.2 Deoxyribonucleic Acid Uncoating and Nuclear Import7. Conclusions and Perspectives; Acknowledgments; References; 3 -- Adenovirus Replication; 1. Introduction; 2. Classification; 3. Adenovirus Genome Organization; 4. Virus Infection; 5. Early Gene Expression; 5.1 Early Region 1A; 5.2 Early Region 1B; 5.3 Early Region 2; 5.4 Early Region 3; 5.5 Early Region 4; 6. Viral DNA Replication; 7. Virus-Associated RNA Genes; 8. Late Gene Expression; 9. Viral DNA Packaging; 10. Conclusion; References; 4 -- Adenoviral Vector Construction I: Mammalian Systems; 1. Introduction; 1.1 Adenovirus Biology. 1.2 Adenovirus Vectors2. Cell Lines for Propagating Adenovirus Vectors; 2.1 Propagation of Adenovirus Vectors Encoding Toxic Transgenes for Cancer Gene Therapy; 3. Construction of First-Generation Adenoviral Vectors; 3.1 Early Methods; 3.2 The Two-Plasmid Rescue System; 3.2.1 Development of the Two-Plasmid Rescue System; 3.2.2 Fine-Tuning of the Two-Plasmid Rescue System; 4. Steps Involved in Adenovirus Vector Construction; 4.1 Preparation of Adenovirus Genomic and Shuttle Plasmid DNA for Cotransfection; 4.2 Cotransfection of HEK-293 Cells with Genomic and Shuttle Plasmid. Gene therapy. Adenoviruses. HEALTH & FITNESS Diseases Genetic. bisacsh MEDICAL Genetics. bisacsh Adenoviruses. fast (OCoLC)fst00796586 Gene therapy. fast (OCoLC)fst00939638 Genetic Therapy methods. Electronic books. Curiel, David, editor. Print version: Adenoviral vectors for gene therapy. [S.l.] : Elsevier Academic Press, 2016 012800276X (OCoLC)932262256 ScienceDirect http://www.sciencedirect.com/science/book/9780128002766 247306 247306