05817cam a2200553Ii 4500 ocn945095030 OCoLC 20190328114814.0 m o d cr cnu|||unuuu 160318t20162016ne a ob 001 0 eng d N$T eng rda pn N$T N$T YDXCP N$T OPELS OCLCF CDX COO EBLCP IDEBK IDB DEBSZ CNCGM MERUC OCLCQ OCLCO U3W UUM WRM D6H ORE OCLCQ 945567083 945613192 9780128016848 (electronic bk.) 0128016841 (electronic bk.) 012801394X 9780128013946 9780128013946 (OCoLC)945095030 (OCoLC)945567083 (OCoLC)945613192 QH581.2 SCI 056000 bisacsh 571.6 23 Casem, Merri Lynn, author. Case studies in cell biology / [electronic resource] Merri Lynn Casem. Amsterdam : Academic Press is an imprint of Elsevier, [2016] �2016 1 online resource (xix, 384) : illustrations (some color). text txt rdacontent computer c rdamedia online resource cr rdacarrier Problem sets in biological and biomedical sciences Includes bibliographical references and index. Online resource; title from PDF title page (EBSCO, viewed March 23, 2016). "Case Studies in Cell Biology presents real world scenarios to help readers use science process and reasoning skills. The case studies require application and analyzation of concepts beyond rote memory of biological concepts. The book is based on the student learning outcomes from the American Society for Cell Biology, offering practical application for both the classroom and research laboratory. Guides the reader in applying knowledge directly to real world scenarios Includes case studies to bridge foundational cell biological concepts to translational science Aids students in synthesizing information and applying science processes." Cover; Title Page; Copyright Page; Dedication; Contents; Biography; Preface; Chapter 1 -- Introduction to Scientific Method from a Cellular Perspective; Subchapter 1.1 -- Scientific Method in Action [1]; Introduction; Background; Methods; Cell cultures; Microinjection; Electron microscopy; Calculation of nuclear uptake and size distribution; Results; Reference; Chapter 2 -- Cellular Biodiversity; Subchapter 2.1 -- Cellular Biodiversity on the High Seas [1]; Introduction; Background; Methods; Cell culture and population density; Characterization of photosynthetic pigments; Results. Subchapter 2.2 -- The Mystery of the Missing Mitochondria [2]Introduction; Background; Methods; Subcellular fractionation of giardia; Transmission electron immunomicroscopy; Results; References; Chapter 3 -- Proteins; Subchapter 3.1 -- Bite of the Brown Recluse Spider: An Introduction to Protein Gel Electrophoresis [1]; Introduction; Background; Methods; Red blood cell ghosts; Venom digestion assays; Protein gel electrophoresis; Results; Subchapter 3.2 -- Where to Start? A Case of Too Many AUGs [3]; Introduction; Background; Methods; Yeast culture conditions. CDC9-GFP fusions, CDC9 mutagenesis, and HA taggingSubcellular fractionation and protease K digestion; Immunoblotting; Results; Subchapter 3.3 -- Cotranslational Translocation: Gatekeepers of the RER [4]; Introduction; Background; Methods; In vitro translation; Crosslinking assay; Flotation assay; Protease resistance assay; Results; Subchapter 3.4 -- From Sequence to Function [5]; Introduction; Background; Methods; Isolation and structural analysis of bovine AQP1; Data processing; Results; Subchapter 3.5 -- Visualizing Protein Conformation [6]; Introduction; Background; Methods. Protein expression and purificationElectron microscopy; SNARE complex labeling; Results; Subchapter 3.6 -- Second Chance Chaperones: How Misfolded Proteins get Refolded [7]; Introduction; Background; Methods; Purification of Hsp104; Purification of Hsp40 and Hsp70; Preparation of yeast lysates; Turbidity assay; Refolding assays; Gel filtration; Results; References; Chapter 4 -- The Nucleus; Subchapter 4.1 -- Nuclear Pore Complex Assembly is a House of Cards [1]; Introduction; Background; Methods; Nuclear reconstitution assay; Immunodepletion; Nuclear import; Immunoblotting; DNA replication assay. Scanning electron microscopyResults; Subchapter 4.2 -- Importin b: The Important Importin [2]; Introduction; Background; Methods; Preparation of a high-speed supernatant from Xenopus eggs; Immunodepletion of importin from cytosol; Nuclear protein import assay; Nuclear localization signal binding assay; Protein gel electrophoresis and immunoblotting; Results; Subchapter 4.3 -- A Tale of tRNA Transport [3]; Introduction; Background; Methods; In vitro translation and 35S-labeling; Injection into Xenopus frog eggs; In vitro binding assays; Gel electrophoresis and immunoblotting; Results. Subchapter 4.4 -- When It Comes to the Nucleus -- Size Matters [4]. Cytology Case studies. SCIENCE Life Sciences Anatomy & Physiology. bisacsh Cytology. fast (OCoLC)fst00886282 Electronic books. Case studies. fast (OCoLC)fst01423765 Print version: Casem, Merri Lynn. Case studies in cell biology. London, England : Academic Press, �2016 xix, 384 pages Problem Sets in Biological and Biomedical Sciences series 9780128013946 Problem sets in biological and biomedical sciences. ScienceDirect http://www.sciencedirect.com/science/book/9780128013946 247304 247304