05901cam a2200637Ia 4500 ocn881571700 OCoLC 20190328114807.0 m o d cr cnu---unuuu 140623s2014 onc o 000 0 eng d EBLCP eng pn EBLCP OPELS N$T UIU OCLCF OCLCQ DEBSZ DEBBG OCLCQ ITD YDX MERER OCLCQ ESU OCLCQ OCLCO OCL OCLCO MERUC OCLCQ U3W D6H CHVBK UUM OTZ OCLCQ 961202138 1076563831 9780124095366 (electronic bk.) 0124095364 (electronic bk.) 9780124104907 (electronic bk.) 0124104908 (electronic bk.) 9780124095366 0124095364 (OCoLC)881571700 (OCoLC)961202138 (OCoLC)1076563831 QH442.6 QY 25 SCI 013060 bisacsh TEC 009010 bisacsh 660.65 23 Transgenic animal technology : a laboratory handbook / [electronic resource] edited by Carl A. Pinkert. 3rd ed. Burlington : Elsevier Science, 2014. 1 online resource (715 pages). text txt rdacontent computer c rdamedia online resource cr rdacarrier Elsevier insights Print version record. Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer - from molecular methods to whole animal considerations - for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engine. Front Cover; Transgenic Animal Technology; Copyright Page; Contents; List of Contributors; Preface; One: Overview; 1 Introduction to Transgenic Animal Technology; I. Introduction; II. Historical Background; III. Applications and Overview of Text; References; Two: Transgenic Animal Production Focusing on the Mouse Model; 2 DNA Microinjection, Embryo Handling, and Germplasm Preservation; I. Introduction; II. General Methods; A. DNA Preparation and Purification; 1. DNA Construct/Fragment Structure; 2. Factors that Influence Transgenic Mouse Production; 3. Preparation of DNA for Microinjection. A. Construction and Isolation of DNAb. DNA Purification; c. Purification and Quantification of DNA; B. Superovulation, Egg Culture, and Harvest; 1. Mouse Strains for Microinjection and Transgenic Models; a. Strains; b. Genetic Variability and Transgene Expression; 2. Superovulation of Mice; a. Pregnant Mare Serum Gonadotropin and Human Chorionic Gonadotropin; b. Follicle Stimulating Hormone and Luteinizing Hormone Delivered with Osmotic Pumps; 3. Production of Eggs from Superovulated Females; a. Colony; b. Influence of Litter Number; 4. In Vitro Culture of Superovulated Eggs; a. Equipment. B. Atmosphere for Culturing Eggsc. Glass Pipettes for Manipulating Eggs; d. Media; e. Water Sources; f. Culture Dishes; 5. Harvesting Superovulated Eggs; a. Mating; b. Oviduct Dissection; c. Release of Eggs from the Oviduct; d. Egg Development and Microinjection Timing; e. Efficiency of Egg Culture; C. Microinjection Needles and Slides; 1. Types of Glass; 2. Preparation of Glass; 3. Pipette Pullers; 4. Injection Pipette Shapes; 5. Beveling Hollow Capillary Microinjection Needles; 6. Microforge Shaping of Pipettes; a. Manufacturers; b. Heating Filament; c. Holding Pipette; d. Injection Pipette. 7. Microinjection Slide for Eggsa. Slide Designs; b. Microinjection Media; c. Arrangement of DNA and Media Drops; D. Microinjection Equipment; 1. Microscopes; 2. Micromanipulators; a. Micropipette Holders; b. Micromanipulator Adjustments; c. Antivibration Tables; 3. Microinjection Systems; a. General; b. Oil-Driven Injection Assembly; 4. Oil-Driven Holding Pipettes; 5. Electronic Delivery Systems; E. Microinjection Procedure; 1. Preparation of Injection and Holding Pipettes (Micrometer Syringe System); a. Holding Pipette; b. Injection Pipette; c. Aligning the Injection Needle. D. Loading DNA into the Injection Needle2. Preparing Eggs for Microinjection; 3. Steps for Efficient Manipulation of Eggs During Microinjection; a. General; b. Micromanipulators; c. Microinjection Needle; d. Controls and Egg Manipulation; 4. Visualizing and Injecting the Egg Pronuclei; a. Orientation; b. Egg Membrane Penetration with the Injection Needle; c. Filling the Pronuclei with DNA Solution; 5. Criteria for Microinjection to Yield Transgenic Mice; a. Treatment After Microinjection; b. Egg Lysis; c. Record Keeping; d. In Vitro Culture of Microinjected Eggs; F. Egg Transfer. Animal genetic engineering. Transgenic animals. Animal biotechnology. SCIENCE Chemistry Industrial & Technical. bisacsh TECHNOLOGY & ENGINEERING Chemical & Biochemical. bisacsh Animal biotechnology. fast (OCoLC)fst00809103 Animal genetic engineering. fast (OCoLC)fst00809192 Transgenic animals. fast (OCoLC)fst01154666 Animals, Genetically Modified. Electronic books. Laboratory Manuals. Pinkert, Carl A., editor. Erscheint auch als: Druck-Ausgabe Pinkert, Carl A. Transgenic Animal Technology . A Laboratory Handbook Elsevier insights. ScienceDirect http://www.sciencedirect.com/science/book/9780124104907 246920 246920