05582cam a2200589Mi 4500 ocn871642550 OCoLC 20190328114807.0 m o d cr |n||||||||| 140307t20142014enk ob 001 0 eng d IDEBK eng rda pn IDEBK OPELS E7B CDX YDXCP COO CHVBK DEBSZ MEU EBLCP N$T OCLCF OCLCQ OCL OCLCQ OCLCA OCLCO MERUC OCLCO OCLCA U3W OCLCO D6H OTZ OCLCQ 871225500 922150995 9780124167131 (electronic bk.) 0124167136 (electronic bk.) 1306464358 (ebk) 9781306464352 (ebk) 9780124095137 0124095135 (OCoLC)871642550 (OCoLC)871225500 (OCoLC)922150995 QH212.F55 SCI 000000 bisacsh 502.8/2 23 Fluorescence microscopy : super-resolution and other novel techniques / [electronic resource] edited by Anda Cornea and P. Michael Conn. London : Academic/Elsevier Press, 2014. �2014 1 online resource text txt rdacontent computer c rdamedia online resource cr rdacarrier Text in English. Includes index. Print version record. Includes bibliographical references and index. Front Cover; Fluorescence Microscopy: Super-Resolution and Other Novel Techniques; Copyright; Contents; Preface; List of Contributors; Chapter 1 -- Evanescent Excitation and Emission; INTRODUCTION; EVANESCENCE IN GENERAL; EVANESCENCE IN EXCITATION: TIR; EVANESCENCE IN EMISSION: THE FLUOROPHORE NEAR FIELD; EVANESCENCE FROM A POINT SOURCE: NSOM; Acknowledgments; References; Chapter 2 -- Adaptive Optics for Fluorescence Microscopy; INTRODUCTION; ABERRATIONS IN MICROSCOPY; PRINCIPLES OF ADAPTIVE OPTICS; ADAPTIVE ABERRATION CORRECTION IN FLUORESCENCE MICROSCOPES; WAVEFRONT SENSING. SCHEMES FOR MODAL SENSORLESS ADAPTIVE OPTICSCONCLUSION; References; Chapter 3 -- Rapid Measurements of Orientation and Rotation in Ex Vivo Muscle Made Possible by Studying Small Number of Cross-Bridges; INTRODUCTION; KINETICS; DISTRIBUTION OF CROSS-BRIDGE ORIENTATIONS; CONCLUSIONS; Acknowledgments; References; Chapter 4 -- High Spatiotemporal Bioimaging Techniques to Study the Plasma Membrane Nanoscale Organization; INTRODUCTION; SPATIAL RESOLUTION; TEMPORAL RESOLUTION; CONCLUSIONS AND OUTLOOK; References; Chapter 5 -- High-Resolution 3D Imaging of Intact Transparent Organs by 3DISCO. INTRODUCTIONCURRENT TECHNOLOGIES FOR IMAGING THE INTACT BRAIN; 3DISCO; Acknowledgments; References; Chapter 6 -- Using RNA Mimics of GFP to Image RNA Dynamics in Mammalian Cells; INTRODUCTION; STUDYING TRINUCLEOTIDE-REPEAT-CONTAINING RNA; OPTIMIZING IMAGING OF CGG REPEAT-CONTAINING RNA; TIME-LAPSE IMAGING OF CGG-REPEAT-CONTAINING RNAS; MONITORING CGG REPEAT FOCI DURING CELL DIVISION; EXAMINING THE STABILITY OF CGG60 RNA; IMAGING THE EFFECTS OF SMALL-MOLECULE DRUGS ON CGG FOCI; IMAGING OTHER RNAS USING SPINACH2; TROUBLESHOOTING IMAGING WITH SPINACH2-TAGGED CONSTRUCTS; IMAGING RARE RNAS. FUTURE DIRECTIONSReferences; Chapter 7 -- Surface Enhanced Raman Scattering (SERS) Image Cytometry for High-Content Screening; HIGH-CONTENT SCREENING: STATUS AND NEEDS; LABELS AND PROBES FOR HCS: FLUORESCENCE AND SERS; SPECTRAL IMAGING FOR FLUORESCENCE AND SERS; SERS IMAGING IN CELL-BASED ASSAYS; STATUS AND PROSPECTS; Acknowledgments; References; APPENDIX; Chapter 8 -- Light Sheet Fluorescence Microscopy Applications for Multicellular Systems; INTRODUCTION; LSFM IMPLEMENTATIONS: A UNIVERSAL CONCEPT WITH MANY ACRONYMS; LSFM AT THE MACROSCOPIC SCALE; 3D CANCER IMAGING MODEL: MCTS. CALCIUM IMAGING, FAST VOLUMETRIC CALCIUM IMAGINGCONCLUSIONS; Acknowledgments; References; Chapter 9 -- Correlative Light Electron Microscopy as a Navigating Tool for Cryo-Electron Tomography Analysis; INTRODUCTION; ELECTRON MICROSCOPY IN CELLULAR AND STRUCTURAL BIOLOGY; SAMPLE PREPARATION FOR ELECTRON MICROSCOPY; CRYO-ELECTRON TOMOGRAPHY; CORRELATIVE LIGHT AND ELECTRON MICROSCOPY (CLEM); CORRELATED LIGHT AND ELECTRON MICROSCOPY DIRECT OBSERVATION IN CRYOGENIC TEMPERATURES; CLEM PROSPECTS; Acknowledgments; References. Chapter 10 -- Cellular and Molecular Applications of Super-resolution Microscopy. Fluorescence Microscopy: Super-Resolution and other Novel Techniques delivers a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science. With contributions selected for clarity, utility, and reproducibility, the work provides practical tools for investigating these ground-breaking developments. Emphasizing super-resolution techniques, light sheet microscopy, sample preparation, new labels, and analysis techniques, this work keeps pace with the innovative technical advances that are increasingly vital to biological an. Fluorescence microscopy. SCIENCE General. bisacsh Fluorescence microscopy. fast (OCoLC)fst00928103 Fluoreszenzmikroskopie gnd (DE-588)4290958-2 Microscopy, Fluorescence. Electronic books. Cornea, Anda, editor. Conn, P. Michael, editor. Print version: 9781306464352 ScienceDirect http://www.sciencedirect.com/science/book/9780124095137 246884 246884