04976cam a2200697Ii 4500 ocn904046277 OCoLC 20171025094524.0 m o d cr cnu|||unuuu 150226s2015 gw ob 001 0 eng d 9783527687978 (electronic bk.) 3527687971 (electronic bk.) 9783527687992 (electronic bk.) 3527687998 (electronic bk.) 3527335161 9783527335169 9783527687985 (Epub) 352768798X (Epub) 9783527688005 (mobi) 3527688005 (mobi) 9783527335169 AU@ 000055036074 CHBIS 010442430 CHNEW 000705551 CHVBK 334085810 NZ1 16078520 DEBBG BV042991323 DEBBG BV043397557 (OCoLC)904046277 (OCoLC)961696441 (OCoLC)962730044 N$T eng rda pn N$T N$T DG1 E7B OCLCF COO CDX YDXCP IDEBK OCLCQ DEBBG N9V CQ$ EBLCP MAIN TD427.M53 TEC 010000 bisacsh 628.1662 23 Modern techniques for pathogen detection / edited by Jürgen Popp and Michael Bauer. [electronic resource] Weinheim, Germany : Wiley Blackwell, 2015. 1 online resource. text txt rdacontent computer c rdamedia online resource cr rdacarrier Includes index. Includes bibliographical references at the end of each chapters and index. Related Titles; Title Page; Copyright; Table of Contents; Preface; List of Contributors; Chapter 1: Unmet Medical Needs in Life-Threatening Infections -- Caring for the Critically Ill; 1.1 Life Threatening Infections and Sepsis -- Defining the Problem; 1.2 Sepsis as a "Hidden Healthcare Disaster"; 1.3 Microorganisms and Types of Infection Triggering Sepsis; 1.4 Emerging Problems Related to Resistance in Bacterial Infections; 1.5 The Role of Fungi and Viruses; 1.6 The Need for New Approaches in Diagnostics of Life-Threatening Infection and Sepsis 1.7 Rapid and Sensitive Culture-Independent Strategies to Identify Blood Stream Infection1.8 Beyond Infection -- Profiling the Immune Response of the Septic Host; 1.9 Host Factors Contributing to Pathogenesis of Sepsis; References; Chapter 2: Identification Methods -- An Overview; 2.1 Taxonomy of Pathogenic Organisms; 2.2 Microscopic Methods; 2.3 Culture-Based Methods; 2.4 Nucleic Acid-Based Techniques; 2.5 Serology; 2.6 Conclusions and Perspectives; References; Chapter 3: Nucleic Acid Amplification Techniques; 3.1 Introduction; 3.2 The Basic PCR Protocol; 3.3 Primer Design 3.4 Modified End-Point PCR Protocols3.5 Non-PCR NAT: Isothermal Amplification Protocols; 3.6 Quantitative PCR; 3.7 Controls, Probing, and General Aspects of Result Interpretation; 3.8 Preanalytics; 3.9 Fields of PCR Application; 3.10 Microbial Trace Detection in BSI, Ascites, and Synovial Fluids; 3.11 Upcoming Routine Solutions -- Dawn of Assay Automation; 3.12 Conclusion and Perspective; Acknowledgment; References; Chapter 4: DNA Microarrays for Pathogen Detection; 4.1 Introduction; 4.2 DNA Microarrays for the Detection of Bacterial Pathogens; 4.3 Antibiotic Resistance Detection 4.4 DNA Microarrays for Virus Diagnostics4.5 DNA Microarrays for Detection of Fungal Pathogens; 4.6 DNA Microarrays for Parasite Diagnostics; 4.7 Conclusion an Outlook; References; Chapter 5: MALDI-ToF; 5.1 Introduction; 5.2 MALDI-ToF Technology; 5.3 Bacterial Identification Using Mass Spectrometry; 5.4 Culture-Independent Rapid Identification of Clinical Pathogens; 5.5 Antibiotic Susceptibility Testing Using Mass Spectrometry; References; Chapter 6: IR and Raman Spectroscopy for Pathogen Detection; 6.1 Introduction; 6.2 Bulk Analysis; 6.3 Excitation with Visible Wavelengths 6.4 UV-Resonance Raman Spectroscopy6.5 Single-Cell Analyses; 6.6 Conclusion and Outlook; References; Chapter 7: FISH; 7.1 Introduction; 7.2 FISH Techniques; 7.3 Clinical Implications of PNA FISH; 7.4 Summary and Outlook; References; Chapter 8: Conclusions; Index; End User License Agreement Vendor-supplied metadata. Pathogenic microorganisms Detection. TECHNOLOGY & ENGINEERING Environmental General. bisacsh Pathogenic microorganisms Detection. fast (OCoLC)fst01054936 Microorganismos patógenos Identificación. Electronic books. Electronic books. Popp, Jürgen, editor. Bauer, Michael, editor. Print version: 9783527687978 (OCoLC)904046277 http://onlinelibrary.wiley.com/book/10.1002/9783527687978 Wiley Online Library ddc BK 207905 207905