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  <titleInfo>
    <title>Insect histology : practical laboratory techniques</title>
  </titleInfo>
  <name type="personal">
    <namePart>Barbosa, Pedro</namePart>
    <namePart type="date">1944-</namePart>
    <role>
      <roleTerm authority="marcrelator" type="text">creator</roleTerm>
    </role>
  </name>
  <name type="personal">
    <namePart>Berry, Deborah L.</namePart>
    <namePart type="date">1972-</namePart>
  </name>
  <name type="personal">
    <namePart>Kary, Christina S.</namePart>
    <namePart type="date">1975-</namePart>
  </name>
  <typeOfResource>text</typeOfResource>
  <genre authority="marc">bibliography</genre>
  <genre authority="fast">Laboratory manuals.</genre>
  <genre authority="">Electronic books.</genre>
  <genre authority="">Electronic books.</genre>
  <originInfo>
    <place>
      <placeTerm type="code" authority="marccountry">enk</placeTerm>
    </place>
    <dateIssued encoding="marc">2014</dateIssued>
    <edition>Second edition.</edition>
    <issuance>monographic</issuance>
  </originInfo>
  <language>
    <languageTerm authority="iso639-2b" type="code">eng</languageTerm>
  </language>
  <physicalDescription>
    <form authority="gmd">electronic resource</form>
    <extent>1 online resource.</extent>
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  <abstract>This title is a much needed update of Barbosa's self-published Manual of Basic Techniques in Insect Histology. It is a laboratory manual of 'traditional' and 'modern' insect histology techniques, completely revised using cutting-edge methodology carried out today and includes new immunohistochemical techniques not previously looked at. Insect Histology is designed as a resource for student and professional researchers, in academia and industry, who require basic information on the procedures that are essential for the histological display of the tissues of insects and related organisms.</abstract>
  <tableOfContents>Problems of sclerotized chitin : softening insect cuticle -- Fixation -- Dehydration, clearing, and embedding -- Staining -- Immunohistochemical staining -- Use of genetic markers in insect histology -- Fluorescence -- Mounting -- Preparation of whole mounts -- Whole mount staining -- Preparation of insect tissues -- Preparation of genitalia, mouthparts, and other body parts -- Preparation of chromosomes -- Appendix : dissecting fluids and insect saline solutions.</tableOfContents>
  <note type="statement of responsibility">Pedro Barbosa, Deborah L. Berry, Christina S. Kary.</note>
  <note>Includes bibliographical references and index.</note>
  <subject authority="lcsh">
    <topic>Entomology</topic>
    <topic>Laboratory manuals</topic>
  </subject>
  <subject authority="lcsh">
    <topic>Histology</topic>
    <topic>Laboratory manuals</topic>
  </subject>
  <subject authority="fast">
    <topic>Entomology</topic>
  </subject>
  <subject authority="fast">
    <topic>Histology</topic>
  </subject>
  <subject authority="bisacsh">
    <topic>SCIENCE / Life Sciences / Zoology / General</topic>
  </subject>
  <subject>
    <topic>Histology</topic>
  </subject>
  <classification authority="lcc">QL464</classification>
  <classification authority="ddc" edition="23">595.7</classification>
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    <titleInfo>
      <title>Insect histology</title>
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    <name>
      <namePart>Barbosa, Pedro, 1944-</namePart>
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    <originInfo>
      <publisher>Chichester, West Sussex ; Hoboken, NJ : John Wiley &amp; Sons, Inc., 2014</publisher>
      <edition>Second edition.</edition>
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    <identifier type="local">(DLC)  2013050117</identifier>
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  <identifier type="isbn">9781118875001 (ePub)</identifier>
  <identifier type="isbn">1118875001 (ePub)</identifier>
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  <identifier type="isbn">9781118876114</identifier>
  <identifier type="isbn">1118876113</identifier>
  <identifier type="lccn">2014000681</identifier>
  <identifier type="issue number">EB00589473 Recorded Books</identifier>
  <identifier type="uri">http://onlinelibrary.wiley.com/book/10.1002/9781118876114</identifier>
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    <recordCreationDate encoding="marc">140108</recordCreationDate>
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    <recordIdentifier source="OCoLC">ocn867852793</recordIdentifier>
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