04688cam a2200577Ma 4500001001300000003000600013005001700019006001900036007001500055008004100070020003600111020003300147020003200180020002900212020002700241020002400268029002200292029002100314029002100335029002300356029002100379029001800400029001800418035002100436037001600457040007400473049000900547050001200556060001000568082001600578245010700594260004600701300003400747336002600781337002600807338003600833505060100869505057201470505058402042505059502626505059803221588002603819650002203845650004803867655002203915700002403937776003403961856008403995942001204079999001904091ocn842412695OCoLC20171106115917.0m o d cr |n||||||||a110329s2013 gw fo 000 0 eng d a9783527669417q(electronic bk.) a3527669418q(electronic bk.) a9781299448902q(MyiLibrary) a1299448909q(MyiLibrary) z9783527322831q(Cloth) z3527322833q(Cloth)1 aAU@b0000516294141 aCHBISb0100269231 aCHVBKb30623761X1 aDEBBGbBV0433958201 aGBVCPb7902072731 aNZ1b153401371 aNZ1b15905464 a(OCoLC)842412695 a476140bMIL aCDXbengepncCDXdDG1dYDXCPdNOCdOCLCQdOCLCOdOCLCFdOCLCQdEBLCP aMAIN 4aRM301.4 4aQV 3804a615.1/922200aModern biopharmaceuticals : recent success stories /cedited by Jorg Knablein.h[electronic resource]  aWeinheim :bWiley-VCH Verlag GmbH,c2013. a1 online resource (450 pages) atextbtxt2rdacontent acomputerbc2rdamedia aonline resourcebcr2rdacarrier0 aRelated Titles; Title Page; Copyright; Dedication; Foreword by Andreas Busch; Foreword by Günter Stock; Preface; Quotes; List of Contributors; Part I: Modern Biopharmaceuticals: Research is the Best Medicine -- Sanitas Summum Bonus; Chapter 1: Twenty Thousand Years of Biotech -- From "Traditional" to "Modern Biotechnology"; 1.1 Biotechnology -- The Science Creating Life; 1.2 The Inauguration of Biotechnology; 1.3 From "Traditional" to "Modern Biotechnology"; 1.4 A Small Molecule from Bacteria -- A Huge Importance for Mankind; 1.5 Biopharmaceuticals -- The Mainstay of Modern Biotechnology8 a1.6 Transformation of the Pharma Industry Through Biotechnology1.7 Biopharmaceutical Production -- Uncorking Bottlenecks or Wasting Surplus Capacity?; 1.8 Conclusion and Outlook; References; Part II: Modern Biopharmaceutical Development Using Stem Cells, Tissues, and Whole Animals; Chapter 2: Induced Pluripotency as Substitute of Somatic Cell Nuclear Transfer? -- The Impact of Induced Pluripotent Stem Cells on Drug Discovery and Regenerative Biopharmaceuticals; 2.1 Introduction; 2.2 Derivation and Growth of hESC; 2.3 Signaling Pathways and Transcription Factors8 a2.4 Differentiation and Applications of hESC2.5 Patient-Specific Nuclear Transfer Stem Cells; 2.6 Patient-Specific Pluripotent Cells Through Direct Reprogramming of Adult Somatic Cells; 2.7 Concluding Remarks and Outlook; Acknowledgment; References; Chapter 3: Pluripotent Stem Cell-Derived Cardiomyocytes for Industrial and Clinical Applications; 3.1 Introduction; 3.2 Pluripotent Stem Cells; 3.3 High-Yield Differentiation of Pluripotent Stem Cells into Cardiomyocytes; 3.4 Purification of Pluripotent Stem Cell-Derived Cardiomyocytes; 3.5 Cardiomyocytes at an Industrial Scale8 a3.6 Utilization of Tissue Engineering Technologies to Advance Cellular Maturity3.7 Concluding Remarks; References; Chapter 4: Industrialization of Functional Mouse Genomics Technologies for Biopharmaceutical Drug Discovery and Development; 4.1 Introduction; 4.2 The Mouse Genetics Story; 4.3 Establishing Inducible Gene Targeting Tools; 4.4 RNAi -- Talking About a Revolution?; 4.5 Further Shortening the Generation Timeline for RNAi Mouse Models; 4.6 Adapting the Mouse Genetics Toolbox for New Applications; References; Part III: Innovative Development Tools for Modern Biopharmaceuticals8 aChapter 5: Standardized Solutions for Quantitative and Real-Time RT-PCR to Accelerate Biopharmaceutical Development5.1 Introduction; 5.2 Potential of Real-Time RT-PCR in Biopharmaceutical Development; 5.3 Accurate Gene Expression Analysis Depends on Standardized Preanalytical Steps; 5.4 Accuracy of Real-Time RT-PCR Depends on Efficient cDNA Synthesis; 5.5 Integration of Preanalytical Steps Streamlines Gene Expression Analysis; 5.6 Overview of Methods for Real-Time RT-PCR; 5.7 Developments in Real-Time PCR Instrumentation; 5.8 The Need for Better Standardization of Quantification Methods0 aPrint version record. 0aBiopharmaceutics. 7aBiopharmaceutics.2fast0(OCoLC)fst00832650 4aElectronic books.1 aKnäblein, Jörg.08iPrint version:z978129944890240uhttp://onlinelibrary.wiley.com/book/10.1002/9783527669417zWiley Online Library 2ddccBK c206708d206708