09187cam a2200721Ka 4500 ocn779616894 OCoLC 20171115091308.0 m o d cr cn||||||||| 120309s2012 gw a ob 001 0 eng d 9783527636778 (electronic bk.) 3527636773 (electronic bk.) 9783527636785 (electronic bk.) 3527636781 (electronic bk.) 9783527326068 AU@ 000049014043 DEBBG BV040092838 DEBBG BV041905525 DEBSZ 397224281 DEBSZ 431085293 DEBSZ 449280675 DKDLA 820120-katalog:000600676 NLGGC 382363604 NZ1 14976191 DEBBG BV043394340 (OCoLC)779616894 (OCoLC)773565121 (OCoLC)778314401 (OCoLC)778436256 (OCoLC)817056533 (OCoLC)844915060 (OCoLC)961599857 (OCoLC)962605276 10.1002/9783527636778 Wiley InterScience http://www3.interscience.wiley.com DG1 eng pn DG1 E7B YDXCP N$T OCLCQ OCLCF OCLCQ RIV EBLCP IDEBK DEBSZ UKDOC CN3GA DEBBG OCLCQ NKT AZK MAIN QH450 .A48 2012 SCI 049000 bisacsh 572.88 22 Alternative Pre-mRNA Splicing : Theory and Protocols / edited by Stefan Stamm, Chris Smith, and Reinhard Lührmann. [electronic resource] Weinheim : Wiley-Blackwell, ©2012. 1 online resource (xxxvii, 622 pages) : illustrations (some color) text txt rdacontent computer c rdamedia online resource cr rdacarrier data file rda Bibliography Formerly CIP. Uk Includes bibliographical references and index. Front Matter -- Theory. Splicing in the RNA World / Emanuele Buratti, Maurizio Romano, Francisco E Baralle -- RNPs, Small RNAs, and miRNAs / Michaela Beitzinger, Gunter Meister -- RNA Elements Involved in Splicing / William F Mueller, Klemens J Hertel -- A Structural Biology Perspective of Proteins Involved in Splicing Regulation / Antoine Cľry, Fřďric H-T Allain -- The Spliceosome in Constitutive Splicing / Patrizia Fabrizio, Reinhard Luhrmann -- The Use of to Study the Mechanism of pre-mRNA Splicing / Brian C Rymond -- Challenges in Plant Alternative Splicing / Andrea Barta, Yamile Marquez, John W S Brown -- Alternative Splice Site Selection / Miguel B Coelho, Christopher W J Smith -- Integration of Splicing with Nuclear and Cellular Events / Aparna K Sapra, Karla Neugebauer -- Splicing and Disease / Emanuele Buratti, Francisco E Baralle -- From Bedside to Bench: How to Analyze a Splicing Mutation / Marco Baralle, Diana Baralle -- Basic Methods. Analysis of Common Splicing Problems / Stefan Stamm -- Ultracentrifugation in the Analysis and Purification of Spliceosomes Assembled / Klaus Hartmuth, Maria A van Santen, Reinhard Luhrmann -- Chemical Synthesis of RNA / Claudia H̲bartner -- RNA Interference (siRNA, shRNA) / Daphne S Cabianca, Davide Gabellini -- Expression and Purification of Splicing Proteins / James Sťvenin, Cyril F Bourgeois -- Detection of RNA-Protein Complexes by Electrophoretic Mobility Shift Assay / Manli Shen, Michael G Fried -- Functional Analysis of Large Exonic Sequences Through Iterative Selection / Ravindra N Singh, Natalia N Singh -- Identification of Splicing cis-Elements Through an Ultra-Refined Antisense Microwalk / Natalia N Singh, Joonbae Seo, Ravindra N Singh -- Genomic SELEX to Identify RNA Targets of Plant RNA-Binding Proteins / Olga Bannikova, Maria Kalyna, Andrea Barta -- Detection of Splicing Events. Quantification of Alternative Splice Variants / Miriam Llorian, Christopher W J Smith -- High-Throughput Analysis of Alternative Splicing by RT-PCR / Roscoe Klinck, Benoit Chabot, Sherif Abou Elela -- Monitoring Changes in Plant Alternative Splicing Events / Craig G Simpson, Naeem Hasan Syed, Sujatha Manthri, John D Fuller, Monika Maronova, Branislav Kusenda, Maria Kalyna, Andrea Barta, John WS Brown -- Array Analysis / Pierre de la Grange -- The CLIP Method to Study Protein-RNA Interactions in Intact Cells and Tissues / James Tollervey, Jernej Ule -- RNA₆Protein Crosslinking and Immunoprecipitation (CLIP) in / Branislav Kusenda, Andrea Barta -- Identification of Proteins Bound to RNA / Emanuele Buratti -- Single-Cell Detection of Splicing Events with Fluorescent Splicing Reporters / Hidehito Kuroyanagi, Akihide Takeuchi, Takayuki Nojima, Masatoshi Hagiwara -- Analysis of Splicing . The Preparation of HeLa Cell Nuclear Extracts / Klaus Hartmuth, Maria A van Santen, Tanja R̲sel, Berthold Kastner, Reinhard Luhrmann -- Splicing Assays / Akila Mayeda, Adrian R Krainer -- Assembly and Isolation of Spliceosomal Complexes / Klaus Hartmuth, Maria A van Santen, Peter Odenw̃lder, Reinhard Luhrmann -- Analysis of Site-Specific RNA-Protein Interactions / Nathalie Marmier-Gourrier, Audrey Vautrin, Christiane Branlant, Isabelle Behm-Ansmant -- Immunoprecipitation and Pull-Down of Nuclear Proteins / Natalya Benderska, Chiranthani Sumanasekera, Stefan Stamm -- Analysis of Protein ( -RNA) Complexes by (Quantitative) Mass Spectrometric Analysis / Carla Schmidt, Henning Urlaub -- Fast Cloning of Splicing Reporter Minigenes / Zhaiyi Zhang, Amit Khanna, Stefan Stamm -- Analysis of Splicing Assays / Isabel C L̤pez-Mej̕a, Jamal Tazi -- Coupled Promoter Splicing Systems / Manuel J Munoz, Manuel de la Mata, Alberto R Kornblihtt -- Stable Cell Lines with Splicing Reporters / Christian Kroun Damgaard, Soren Lykke-Andersen, J̜rgen Kjems -- Splicing Factor ChIP and ChRIP: Detection of Splicing and Splicing Factors at Genes by Chromatin Immunoprecipitation / Aparna K Sapra, Fernando Carrillo Oesterreich, Marta Pabis, Imke Listerman, Nicole Bardehle, Karla M Neugebauer -- Yeast Genetics to Investigate the Function of Core Pre-mRNA Splicing Factors / Raymond T O Keefe, Jean D Beggs -- Analysis of HIV-1 RNA Splicing / Simon Duffy, Alan Cochrane -- Analysis of Plant Intron Splicing / Craig G Simpson, Michele Liney, Diane Davidson, Dominika Lewandowska, Maria Kalyna, Sean Chapman, Andrea Barta, John W S Brown -- Modification State-Specific Antibodies / Jordan B Fishman, Olga Kelemen, Eric A Berg -- Analysis of Alternative Splicing in Genetic Mosaics / Shihuang Su, Diana O'Day, Shanzhi Wang, William Mattox -- Manipulation of Splicing Events. Antisense Derivatives of U7 Small Nuclear RNA as Modulators of Pre-mRNA Splicing / Kathrin Meyer, Daniel Schumperli -- Screening for Alternative Splicing Modulators / Peter Stoilov -- Use of Oligonucleotides to Change Splicing / Annemieke Aartsma-Rus, Judith C T van Deutekom -- Changing Signals to the Spliceosome / Denise R Cooper, Niketa A Patel -- Bioinformatic Analysis of Splicing. Overview of Splicing Relevant Databases / Pierre de la Grange -- Analysis of RNA Transcripts by High-Throughput RNA Sequencing / Paolo Ribeca, Vincent Lacroix, Michael Sammeth, Roderic Guig̤ -- Identification of Splicing Factor Target Genes by High-Throughput Sequencing / Chaolin Zhang, Michael Q Zhang -- Bioinformatic Analysis of Splicing Events / Zhaiyi Zhang, Stefan Stamm -- Analysis of Pre-mRNA Secondary Structures and Alternative Splicing / Michael Hiller -- Structure Prediction for Alternatively Spliced Proteins / Lukasz Kozlowski, Jerzy Orlowski, Janusz M Bujnicki -- Comparative Genomics Methods for the Prediction of Small RNA-Binding Sites / Rym Kachouri-Lafond, Mihaela Zavolan -- Appendix A1: Yeast Nomenclature Systematic Open Reading Frame (ORF) and Other Genetic Designations -- Appendix A2: Glossary -- Index. This book was written for graduate and medical students, as well as clinicians and postdoctoral researchers. It describes the theory of alternative pre-mRNA splicing intwelve introductory chapters and then introduces protocols and their theoretical background relevant for experimental research. These 43 practical chapters cover: Basic methods, Detection of splicing events, Analysis of alternative pre-mRNA splicing in vitro and in vivo, Manipulation of splicing events, and Bioinformatic analysis of alternative splicing. A theoretical introduction and practical guide for molecular biologi. Print version record. RNA splicing. Exons (Genetics) Genetic recombination. Split genes. SCIENCE Life Sciences Molecular Biology. bisacsh RNA splicing. fast (OCoLC)fst01086257 Empalmament (Genètica) lemac Biologia molecular. lemac Llibres electrònics. Electronic books. Stamm, Stefan. Smith, Christopher W. J. Lührmann, Reinhard. Print version: Alternative pre-mRNA splicing. Weinheim : Wiley-Blackwell, ©2012 9783527326068 (OCoLC)776877424 http://onlinelibrary.wiley.com/book/10.1002/9783527636778 Wiley Online Library ddc BK 205702 205702