04554cam a2200733Ma 4500 ocn769189204 OCoLC 20171115123136.0 m o d cr cn||||||||| 110927s2012 njua ob 001 0 eng d 2011040418 9781118147146 (electronic bk.) 1118147146 (electronic bk.) 0470610743 9780470610749 9781118147177 (electronic bk.) 1118147170 (electronic bk.) 9781118147160 (electronic bk.) 1118147162 (electronic bk.) 9780470610749 EB00062766 Recorded Books AU@ 000053284818 DEBBG BV040092110 DEBBG BV041910619 DEBBG BV042796238 DEBSZ 372823750 DEBSZ 431068704 DKDLA 820120-katalog:000588044 NZ1 14693823 DEBBG BV043393903 (OCoLC)769189204 (OCoLC)761321891 (OCoLC)762100530 (OCoLC)816871889 (OCoLC)860516270 (OCoLC)864906067 (OCoLC)961637134 (OCoLC)962667987 10.1002/9781118147177 Wiley InterScience http://www3.interscience.wiley.com E7B eng pn E7B YDXCP N$T REDDC OCLCQ DEBSZ OCLCQ OSU OCLCQ OCLCF OCLCQ DG1 IDEBK CDX NTE EBLCP MERUC RECBK NLGGC NLE DEBBG OCLCQ COO OCLCQ AZK MAIN QH588.S83 P75 2012eb QU 325 MED 106000 bisacsh 616.02774 23 Primary and stem cells : gene transfer technologies and applications / edited by Uma Lakshmipathy, Bhaskar Thyagarajan. [electronic resource] Hoboken, NJ : John Wiley and Sons, ©2012. 1 online resource (xiii, 279 pages) : illustrations text txt rdacontent computer c rdamedia online resource cr rdacarrier Includes bibliographical references and index. DNA assembly technologies based on homologus recombination / Billyana Tsvetanova [and others] -- Multigene assembly for construction of synthetic operons : creation and delivery of an optimized all-in-one expression construct for generating mouse iPS cells / Takefumi Sone [and others] -- Strategies for the delivery of naked DNA / Jennifer C. Moore and Ronald P. Hart -- Episomal vectors / Junying Yu -- Nonintegrating DNA virus / Chris Kemp and David Kuninger -- Nonintegrating RNA viruses / Noemi Fusaki -- Protein delivery / Xiao-Jian Han and Kazuhito and Tomizawa -- Sleeping beauty transposon-mediated stable gene delivery / Andrew Wilber [and others] -- Integrating viral vectors for gene modifcations / Gemma L. Mendel, Brian Paszkiet, and Rafal P. Witek -- Bacteriophase integrases for site-specific integration / Bhaskar Thyagarajan -- Improving gene targeting efficiency in human pluripotent stem cells / Sangyoon Han [and others] -- Modifed stem cells as disease models and in toxicity screening / Catharina Ellerstrom [and others] -- Screening and drug discovery / Elizabeth A. Davenport and Robert S. Ames. "This book describes basic cell engineering methods, emphasizing stem cell applications, and use of the genetically modified stem cells in cell therapy and drug discovery. Together, the chapters introduce and offer insights on new techniques for engineering of stem cells and the delivery of transgenes into stem cells via various viral and non-viral systems. The book offers a guide to the types of manipulations currently available to create genetically engineered stem cells that suit any investigator's purpose, whether it's basic science investigation, creation of disease models and screens, or cells for therapeutic applications"--Provided by publisher. Cells. Stem cells. Gene Transfer Techniques. Stem Cells. Genetic Engineering. Transgenes. MEDICAL Research. bisacsh Cells. fast (OCoLC)fst00850250 Stem cells. fast (OCoLC)fst01132975 Electronic books. Lakshmipathy, Uma. Thyagarajan, Bhaskar. Print version: Primary and stem cells. Hoboken, NJ : John Wiley and Sons, ©2012 (DLC) 2011040418 http://onlinelibrary.wiley.com/book/10.1002/9781118147177 Wiley Online Library ddc BK 205468 205468