04167cam a2200625Ia 4500 ocn716174422 OCoLC 20171119085914.0 m o d cr mn||||||||| 110428s2011 enkaf ob 001 0 eng d 015638936 Uk 9780470669822 (electronic bk.) 0470669829 (electronic bk.) 9780470669815 (electronic bk.) 0470669810 (electronic bk.) 9780470975633 0470975636 9780470666586 (pbk.) 0470666587 (pbk.) 10.1002/9780470669815 doi 9786613239365 AU@ 000047158723 DEBBG BV040076766 DEBBG BV043067297 DEBSZ 372734863 DEBSZ 421528796 NZ1 14256898 (OCoLC)716174422 (OCoLC)711780326 (OCoLC)748937805 UIU eng pn UIU DG1 YDXCP OSU CDX E7B N$T OCLCQ REDDC OCLCQ TEF UKMGB OCLCQ DEBSZ OCLCQ OCLCF OCLCQ MAIN QH585.2 .A565 2011eb SCI 017000 bisacsh 571.6/381 22 Animal cell culture : essential methods / editor, John M. Davis. [electronic resource] Chichester, West Sussex ; Hoboken, NJ : Wiley-Blackwell, 2011. 1 online resource (xxvii, 346 pages, [2] pages of color plates) : illustrations (some color) text txt rdacontent computer c rdamedia online resource cr rdacarrier Includes bibliographical references and index. The cell culture laboratory / Sue Clarke and Janette Dillon -- Sterilization / Peter L. Roberts -- Microscopy of living cells / Colin Gray and Daniel Zicha -- Basic techniques and media, the maintenance of cell lines, and safety / John M. Davis -- Development and optimization of serum- and protein-free culture media / Stephen F. Gorfien and David W. Jayme -- Cryopreservation and banking of cell lines / Glyn N. Stacey, Ross Hawkins and Roland A. Fleck -- Primary culture of specific cell types and the establishment of cell lines / Kee Woei Ng [and others] -- Cloning / John Clarke, Alison Porter and John M. Davis -- The quality control of animal cell lines and the prevention, detection and cure of contamination / Peter Thraves and Cathy Rowe -- Systems for cell culture scale-up / Jennifer Halsall and John M. Davis -- Good laboratory practice in the cell culture laboratory / Barbara Orton. Abstract: Animal cell culture is a widely used technology for producing recombinant proteins. The ability to make post-translational modifications and secrete the active forms of the protein into the culture medium represents major advantages over other processes. The growing market demand for pharmaceuticals has created a need for increased production capacity; however, achieving productivity gains in both the upstream stage and downstream processes can subject cells to aggressive environments such as those involving hydrodynamic stresses. Although numerous studies have explored the consequences of cell damage due to hydrodynamic stress, there has been a lack of understanding of the mechanism of such damage at a cellular level. Cell damage can also influence biomedical applications. Cells manipulated in instruments such as diagnosis and analysis devices can experience hydrodynamic forces. Print version record. Cell culture Technique. Tissue culture Technique. SCIENCE Life Sciences Cell Biology. bisacsh Cell culture Technique. fast (OCoLC)fst00850177 Tissue culture Technique. fast (OCoLC)fst01151483 Electronic books. Davis, John, 1952- Print version: Animal cell culture. Hoboken, NJ : John Wiley & Sons Inc., 2011 9780470666586 (DLC) 2010037008 (OCoLC)664450733 http://onlinelibrary.wiley.com/book/10.1002/9780470669815 Wiley Online Library ddc BK 205013 205013