Transgenic animal technology : a laboratory handbook / (Record no. 246920)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 05901cam a2200637Ia 4500 |
| 001 - CONTROL NUMBER | |
| control field | ocn881571700 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OCoLC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20190328114807.0 |
| 006 - FIXED-LENGTH DATA ELEMENTS--ADDITIONAL MATERIAL CHARACTERISTICS | |
| fixed length control field | m o d |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION | |
| fixed length control field | cr cnu---unuuu |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 140623s2014 onc o 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EBLCP |
| Language of cataloging | eng |
| Description conventions | pn |
| Transcribing agency | EBLCP |
| Modifying agency | OPELS |
| -- | N$T |
| -- | UIU |
| -- | OCLCF |
| -- | OCLCQ |
| -- | DEBSZ |
| -- | DEBBG |
| -- | OCLCQ |
| -- | ITD |
| -- | YDX |
| -- | MERER |
| -- | OCLCQ |
| -- | ESU |
| -- | OCLCQ |
| -- | OCLCO |
| -- | OCL |
| -- | OCLCO |
| -- | MERUC |
| -- | OCLCQ |
| -- | U3W |
| -- | D6H |
| -- | CHVBK |
| -- | UUM |
| -- | OTZ |
| -- | OCLCQ |
| 019 ## - | |
| -- | 961202138 |
| -- | 1076563831 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9780124095366 |
| Qualifying information | (electronic bk.) |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 0124095364 |
| Qualifying information | (electronic bk.) |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9780124104907 |
| Qualifying information | (electronic bk.) |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 0124104908 |
| Qualifying information | (electronic bk.) |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| Canceled/invalid ISBN | 9780124095366 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| Canceled/invalid ISBN | 0124095364 |
| 035 ## - SYSTEM CONTROL NUMBER | |
| System control number | (OCoLC)881571700 |
| Canceled/invalid control number | (OCoLC)961202138 |
| -- | (OCoLC)1076563831 |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER | |
| Classification number | QH442.6 |
| 060 #4 - NATIONAL LIBRARY OF MEDICINE CALL NUMBER | |
| Classification number | QY 25 |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | SCI |
| Subject category code subdivision | 013060 |
| Source | bisacsh |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | TEC |
| Subject category code subdivision | 009010 |
| Source | bisacsh |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 660.65 |
| Edition number | 23 |
| 245 00 - TITLE STATEMENT | |
| Title | Transgenic animal technology : a laboratory handbook / |
| Medium | [electronic resource] |
| Statement of responsibility, etc. | edited by Carl A. Pinkert. |
| 250 ## - EDITION STATEMENT | |
| Edition statement | 3rd ed. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | Burlington : |
| Name of publisher, distributor, etc. | Elsevier Science, |
| Date of publication, distribution, etc. | 2014. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 1 online resource (715 pages). |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Content type code | txt |
| Source | rdacontent |
| 337 ## - MEDIA TYPE | |
| Media type term | computer |
| Media type code | c |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | online resource |
| Carrier type code | cr |
| Source | rdacarrier |
| 490 1# - SERIES STATEMENT | |
| Series statement | Elsevier insights |
| 588 0# - SOURCE OF DESCRIPTION NOTE | |
| Source of description note | Print version record. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer - from molecular methods to whole animal considerations - for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engine. |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Front Cover; Transgenic Animal Technology; Copyright Page; Contents; List of Contributors; Preface; One: Overview; 1 Introduction to Transgenic Animal Technology; I. Introduction; II. Historical Background; III. Applications and Overview of Text; References; Two: Transgenic Animal Production Focusing on the Mouse Model; 2 DNA Microinjection, Embryo Handling, and Germplasm Preservation; I. Introduction; II. General Methods; A. DNA Preparation and Purification; 1. DNA Construct/Fragment Structure; 2. Factors that Influence Transgenic Mouse Production; 3. Preparation of DNA for Microinjection. |
| 505 8# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | A. Construction and Isolation of DNAb. DNA Purification; c. Purification and Quantification of DNA; B. Superovulation, Egg Culture, and Harvest; 1. Mouse Strains for Microinjection and Transgenic Models; a. Strains; b. Genetic Variability and Transgene Expression; 2. Superovulation of Mice; a. Pregnant Mare Serum Gonadotropin and Human Chorionic Gonadotropin; b. Follicle Stimulating Hormone and Luteinizing Hormone Delivered with Osmotic Pumps; 3. Production of Eggs from Superovulated Females; a. Colony; b. Influence of Litter Number; 4. In Vitro Culture of Superovulated Eggs; a. Equipment. |
| 505 8# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | B. Atmosphere for Culturing Eggsc. Glass Pipettes for Manipulating Eggs; d. Media; e. Water Sources; f. Culture Dishes; 5. Harvesting Superovulated Eggs; a. Mating; b. Oviduct Dissection; c. Release of Eggs from the Oviduct; d. Egg Development and Microinjection Timing; e. Efficiency of Egg Culture; C. Microinjection Needles and Slides; 1. Types of Glass; 2. Preparation of Glass; 3. Pipette Pullers; 4. Injection Pipette Shapes; 5. Beveling Hollow Capillary Microinjection Needles; 6. Microforge Shaping of Pipettes; a. Manufacturers; b. Heating Filament; c. Holding Pipette; d. Injection Pipette. |
| 505 8# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | 7. Microinjection Slide for Eggsa. Slide Designs; b. Microinjection Media; c. Arrangement of DNA and Media Drops; D. Microinjection Equipment; 1. Microscopes; 2. Micromanipulators; a. Micropipette Holders; b. Micromanipulator Adjustments; c. Antivibration Tables; 3. Microinjection Systems; a. General; b. Oil-Driven Injection Assembly; 4. Oil-Driven Holding Pipettes; 5. Electronic Delivery Systems; E. Microinjection Procedure; 1. Preparation of Injection and Holding Pipettes (Micrometer Syringe System); a. Holding Pipette; b. Injection Pipette; c. Aligning the Injection Needle. |
| 505 8# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | D. Loading DNA into the Injection Needle2. Preparing Eggs for Microinjection; 3. Steps for Efficient Manipulation of Eggs During Microinjection; a. General; b. Micromanipulators; c. Microinjection Needle; d. Controls and Egg Manipulation; 4. Visualizing and Injecting the Egg Pronuclei; a. Orientation; b. Egg Membrane Penetration with the Injection Needle; c. Filling the Pronuclei with DNA Solution; 5. Criteria for Microinjection to Yield Transgenic Mice; a. Treatment After Microinjection; b. Egg Lysis; c. Record Keeping; d. In Vitro Culture of Microinjected Eggs; F. Egg Transfer. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Animal genetic engineering. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Transgenic animals. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Animal biotechnology. |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | SCIENCE |
| General subdivision | Chemistry |
| -- | Industrial & Technical. |
| Source of heading or term | bisacsh |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | TECHNOLOGY & ENGINEERING |
| General subdivision | Chemical & Biochemical. |
| Source of heading or term | bisacsh |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Animal biotechnology. |
| Source of heading or term | fast |
| Authority record control number | (OCoLC)fst00809103 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Animal genetic engineering. |
| Source of heading or term | fast |
| Authority record control number | (OCoLC)fst00809192 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Transgenic animals. |
| Source of heading or term | fast |
| Authority record control number | (OCoLC)fst01154666 |
| 650 #2 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Animals, Genetically Modified. |
| 655 #4 - INDEX TERM--GENRE/FORM | |
| Genre/form data or focus term | Electronic books. |
| 655 #2 - INDEX TERM--GENRE/FORM | |
| Genre/form data or focus term | Laboratory Manuals. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Pinkert, Carl A., |
| Relator term | editor. |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Erscheint auch als: |
| Note | Druck-Ausgabe |
| Main entry heading | Pinkert, Carl A. Transgenic Animal Technology . |
| Title | A Laboratory Handbook |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE | |
| Uniform title | Elsevier insights. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Materials specified | ScienceDirect |
| Uniform Resource Identifier | http://www.sciencedirect.com/science/book/9780124104907 |
No items available.